PRACTICAL APPLICATIONS: Hepatocellular carcinoma (HCC) is among the leading causes of cancer‐related mortality. auricula is a potent inducer of apoptosis in HCC cells via PRDX1‐inhibition pathways. GSH and SOD levels were decreased in the treated cells and We investigated the level of anti‐oxidative enzymes, total glutathione (GSSG/GSH), and superoxide dismutase (SOD) levels in treated cells and PRDX1 gene‐silenced cells. auricula‐induced cell death and anti‐oxidative enzyme. In order to obtain a more validation of the correlation of A. auricula treatment in mRNA and protein level. We confirmed downregulation of expression of PRDX1 following A. Among seven identified proteins, we focused on peroxiredoxin1 (PRDX1), which has been known as an anti‐oxidative enzyme. For more investigation, a two‐dimensional electrophoresis based‐proteomic analysis (2DE‐GE) was employed for identification of possible target‐related proteins of A. Following experiments, we founded that its antiproliferative effects was related with apoptosis‐inducing activities. auricula has most dramatic antiproliferative effects on huh‐7 cell line. Among five cancer cell lines, we focused on huh‐7 cell line, HCC cell line, due to that A. auricula has an antiproliferative effect on various cancer cell lines. First, using proliferative assay including MTT assay and real‐time cell electronic sensing technique, we founded that A. auricula‐induced anticancer activity in hepatocellular carcinoma (HCC) cells. Therefore, this study was conducted to explore possible mechanisms of A. auricula) has been reported to have positive health effects. Therefore, the potentials of targeting apoptosis would be a viable therapeutic strategy to improve the outcome of HCC patients.Īuricularia auricula (A. Regulation of PRDX1 in HCC may contribute to cancer treatment. auricula is an apoptotic inducer of HCC cells through PRDX1 pathway. PRDX1 is currently being investigated as a new target for gene therapy in cancer.
Overexpression of PRDX1 in cancer cells implies the role of PRDX1 in the cancer therapy. PRDX1 has a proliferative effect and play a role in cancer development or progression. In this research article, we provide regulation mechanism of PRDX1 in HCC. Newer treatments are needed with several being in development. Chemotherapy and radiotherapy are generally ineffective. However, in most patients with HCC the diagnosis is often late, thereby excluding the patients from definitive surgical resection. The principal treatment is surgical resection or liver transplantation. GSH and SOD levels were decreased in the treated cells and PRDX1 gene‐silenced cells.
0 kommentar(er)
